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Isolation and Identification of Cancer Stem Cells from Human Osteosarcom by Serum-free Three-dimensi

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摘 要:The cancer stem cells(CSCs)from human osteosarcoma by serum-free three-dimensional culture combined
  • 【题 名】Isolation and Identification of Cancer Stem Cells from Human Osteosarcom by Serum-free Three-dimensional Culture Combined with Anticancer Drugs
  • 【作 者】周松 李锋 肖骏 熊伟 方忠 陈文坚 牛鹏彦
  • 【机 构】Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology
  • 【刊 名】《华中科技大学学报:医学英德文版》2010年 第1期 -页 共4页
  • 【关键词】
  • 【文 摘】The cancer stem cells(CSCs)from human osteosarcoma by serum-free three-dimensional culture combined with anticancer drugs were isolated and identified.The primary cells derived from human osteosarcoma were digested by trypsin to prepare a single-cell suspension,and mixed homogeneously into 1.2% alginate gel.Single-cell alginate gel was cultured with serum-free DMEM/F12 medium.Epirubicin(0.8μg/mL)was added to the medium to enrich CSCs.After cultured conventionally for 7 to 10 days,most of cells suspended in ...
 
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