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Influence of Osteopontin Short Hairpin RNA on the Proliferation and Invasion of Human Renal Cancer C

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摘 要:The influence of short hairpin RNA(shRNA)-mediated osteopontin(OPN)gene silencing on the proliferati
  • 【题 名】Influence of Osteopontin Short Hairpin RNA on the Proliferation and Invasion of Human Renal Cancer Cells
  • 【作 者】柳昊 陈安民 郭风劲 袁林
  • 【机 构】Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology
  • 【刊 名】《华中科技大学学报:医学英德文版》2010年 第1期 -页 共8页
  • 【关键词】
  • 【文 摘】The influence of short hairpin RNA(shRNA)-mediated osteopontin(OPN)gene silencing on the proliferation and invasion of human renal cancer ACHN cells was investigated.Four types of OPN shRNA recombinant plasmids were constructed and RT-PCR assays were used to screen the most highly functional shRNA recombinant plasmids,which were transferred into the cultured ACHN cells by LipofectamineTM 2000.The cells transfected by shRNA expression vectors(ACHN/OPN)were visualized under an inverted microscope and screened...
 
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